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5-Amino-1MQ selectively inhibits nicotinamide N-methyltransferase (NNMT), a methyltransferase enzyme that catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM) to nicotinamide, producing 1-methylnicotinamide

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By inhibiting NNMT, 5-Amino-1MQ has been used to study: How modulation of NNMT affects NAD levels in cell lines and animal models The downstream impact on mitochondrial respiration and oxidative metabolism The relationship between NNMT activity and regulators such as SIRT1, which is widely examined in the context of cellular aging, metabolic control, and stress responses These investigations are mechanistic in nature and are designed to clarify how NNMT fits into broader metabolic and epigenetic networks