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The tissues collected from each group were collected in 0.01 M PBS with protease inhibitors [2 g/mL aprotinin, leupeptin, pepstatin A, and 120 g/mL phenylmethanesulfonyl fluoride (PMSF)] and homogenized (Polytron RT MR3100) at 3 10-s intervals

the mouse study used dosing three times daily

Versus full-length growth hormone Full-length human growth hormone causes numerous side effects that AOD 9604 specifically avoids: Blood sugar disruption : GH impairs insulin sensitivity and can precipitate diabetes in susceptible individuals

Semaglutide requires weekly injections and often causes gastrointestinal side effects, while AOD-9604 is generally well-tolerated with minimal digestive impact

Prog Neuropsychopharmacol Biol Psychiatry 81:5054