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The cells were cultured in Dulbeccos Modified Eagles Medium/Nutrient Mixture F-12 (DMEM/F12 = 1:1) supplemented with 5% (v/v) fetal bovine serum, 1 mM pyruvate, 50 g/mL penicillin, 50 g/mL streptomycin, 10 g/mL insulin, 5.5 g/mL transferrin, 5 ng/mL sodium selenite, and 5 ng/mL epidermal growth factor and maintained at 37 C in a sterile, humidified atmosphere of 5% CO 2

However, it is also possible to observe apical topography and cilia beating in vitro, in real-time, using reductionist approaches such as brightfield microscopy and image analysis

[caption id="attachment_65586" align="alignnone" width="800"] Food-with-the-highest-glutathione[/caption] Dietary Supplements for Boosting Glutathione In addition to implementing lifestyle changes, dietary supplements can also play a role in boosting glutathione levels

doi: 10.1016/j.ymgmr.2013.12.007 91 MahajanASidiropoulosC

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