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Additionally, NAD serves as a substrate for sirtuins, a family of proteins that influence gene expression, DNA repair, and metabolic processes

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Following the importation of offline MS data into Compound Discoverer 3.3 (ThermoFisher Scientific), analysis using the BGI Metabolome Database (BMDB), mzCloud database, and ChemSpider online database was conducted to generate a comprehensive data matrix that included details, such as metabolite peak areas and identification results