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The central challenge for this fungal pretreatment, however, is economic: the process is often slower and less amenable to continuous industrial operation than established physicochemical methods (e.g., steam explosion, acid hydrolysis), raising concerns about capital costs and volumetric productivity at scale (Zhu et al., 2024)

The resulting protein samples were denatured using 100 l of 2 SDS loading buffer and separated via 12% SDSPAGE

doi: 10.1002/humu.22373 182 WenSAkiTUnumaKUemuraK

Additionally, transcriptomic high-throughput sequencing was utilized to acquire the post-treatment inflammatory transcriptomic profile and reveal key regulatory pathways and mechanisms of action

engineered a library of LNPs using orthogonal design of experiments to evaluate the effect of LNP structure on their stability in amniotic fluid and to enable intra-amniotic mRNA delivery in utero (Swingle et al., 2022)