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Available from: [DOI] [PMC free article] [PubMed] [Google Scholar] 123.Donovan A, Brownlie A, Zhou Y, Shepard J, Pratt SJ, Moynihan J, et al

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After several washes in the same buffer, the samples were post-fixed for 60 minutes with 2% osmium tetroxide, washed in deionised water, dehydrated through graded ethanol (70, 95, and 100%) and embedded in epon for 48 hours at 60 C