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DNA was extracted from the tissue by incubating the ear sample in 1 solution A (Solution A (10): 250 mM NaOH, 2 mM EDTA, in water) at 95 C for 30 min, followed by neutralizing the reaction with 1 solution B (Solution B (10): 400 mM Tris-HCl, in water)

Ca2+ influx regulates BDNF transcription by a CREB family transcription factor-dependent mechanism

Qin X, Yang M, Yu Y, Wang X, Zheng Y, Cai R, et al

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Although these concentrations are lower than GSH levels, they suggest that NAC possesses some degree of direct antioxidant activity (Pedre et al., 2021)