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An experiment using human peripheral blood macrophages (M) showed that the intracellular GSH content was dose-dependently increased by treatment of M with cystine, and was increased further by the addition of glutamate (Rimaniol et al

Treatment of cells Hepatoma HepG2 cells were maintained in DMEM (GE Healthcare, Buckinghamshire, UK) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 g/ml streptomycin at 37 C in 5% CO 2

Sakai, et al

The peptides effects on ligament injuries parallel its tendon benefits

336 Several underlying factors, such as differences in the experimental design, model systems, or patient heterogeneity, may account for the differences in the mechanisms of action of CP31398 and APR-246 and cause different therapeutic responses