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Seahorse XFe96/XF Pro Cell Culture Microplates (Agilent Technologies) were coated with poly-D-lysine (Gibco TM ) following the manufacturers protocol

Metformins AMPK-dependent actions also extend to cardiopulmonary I/R models, where it reduces oxidative stress, suppresses inflammatory cytokines (TNF-, IL-1), and inhibits NF-B signaling, collectively preserving tissue integrity (Liu et al., 2024)

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Limited environmental serine and glycine confer brain metastasis sensitivity to PHGDH inhibition

Hydroxyl radicals react with DNA and lipids, causing DNA damage and lipid peroxidation, respectively