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The latter is now commercially unavailable but a solution of the same density and reduced osmolality can be produced from OptiPrep by dilution with a reduced osmolality saline solution see Mononuclear cells Application Sheet C43 in index for more information) Solutions can be prepared as follows: use 0.7% (w/v) NaCl, 10 mM Tricine, pH 7.0 to dilute OptiPrep to produce solutions of 1.050, 1.080, 1.090 g/ml (approx

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