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The following day, coverslips were washed and probed with fluorescently labeled secondary antibodies in 3% BSA for 1 h at room temperature, and then counterstained with 1 g/mL DAPI for 15 min

In this procedure, 2.5 mM H 2 O 2 in 50 nM potassium phosphate buffer (1 mM GSH, 0.1 M NADPH, 0.5 U/mL GR, 1 mM EDTA, 2 mM sodium azide, pH 7.4) was used as the substrate

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doi:10.1124/mol.65.5.1181