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7b), a known inhibitor of the unplugging action of puromycin [33]

Red blood cells were lysed with 500 l of 1 BD FACS lysing solution (BD, 349202) for 5 min at room temperature, stopped with 3 ml of PBS, and stained with the antibody mixture consisting of Mac1, Gr1, B220, and CD3 for 15 min at 4 C, followed by flow cytometry analysis (BD, Fortessa)