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The effects of vitamin D supplementation on biomarkers of inflammation and oxidative stress among women with polycystic ovary syndrome: A systematic review and meta-analysis of randomized controlled trials

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Specific activities have previously been reported for commercial enzyme preparations of GST M1-1 (3.7 mol min 1 mg 1 ), GST A1-1 (14.9 mol min 1 mg 1 ), and GST P1-1 (0.15 mol min 1 mg 1 ) at pH 6.5 and 25 C 8 , but later redetermined with somewhat altered conditions of pH 7.4 and 37 C to the higher values of 66.3, 126, and 1.36 mol min 1 mg 1 , respectively 17 The latter values are in general agreement with the values in Table 1 considering differences in reaction temperature and other conditions

The pellets were resuspended in phosphate-buffered saline (PBS) containing 0.1% hyaluronidase (Sigma-Aldrich, MO, USA) at 37 C for 20 min