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Western blot analysis Cell lysates were prepared using radioimmunoprecipitation assay (RIPA) buffer (Servicebio, Wuhan, China), supplemented with a protease inhibitor cocktail (Roche, IN, USA)

Hepatic injury related to antithyroid therapy such as methimazole is well documented in humans and reported in the cat.22, 32 Mild histologic changes are common, but cases of fulminant hepatic failure with central lobular necrosis have been described

While extrapolated data from UK/US, CF clinics suggest a prevalence of 1:12,0001:40,000 among Indian children (95), a 2020 North Indian study revealed a high CFTR mutation carrier rate of 1:22, signaling a potentially higher prevalence (96)

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