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Under metabolic stress, fatty acids are mobilized from lipid droplets through lipolysis, mediated by lipases, or lipophagy, a selective form of autophagy that degrades lipid droplets (63)

Observing the model after simulating several initial values and varying limiting agents, we found that pyruvate supply is the limiting substrate of the overall pathway

The protease cleaves the fusion protein in vivo , offering the advantage of not compromising the target proteins purity level or its production yields like often occurs in in vitro cleavage strategies (Kapust and Waugh, 2000)

doi: 10.1016/j.livres.2023.10.002