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[9] Pasteur and the discovery of enantiomers In 1848, thirty-three years after Biots work, studies on the optical activity of molecules led Louis Pasteur, who had been a student of Biot, to observe that, following the recrystallization of a concentrated aqueous solution of sodium ammonium tartrate (which was optically inactive), two types of crystals precipitated that were non-superimposable mirror images of each other