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Raw data Raw RNA sequencing data were demultiplexed and converted to FASTQ format using Illuminas Consensus Assessment of sequence and Variation (CASAVA) ( FASTQ files were aligned to the reference genome with STAR (two-pass mode) 56 , and TopHat 57 , and transcript quantification was performed using Cufflinks 58

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Similar to reactive carbonyl species and other reactive glycotoxins, the influence of dAGEs on gut microbiota is suggested to depend on conditions and to vary with the ability of bacteria to metabolize these poorly degradable complexes (103)

9.4 GSH-mediated immunotherapy Cancer immunotherapies, such as vaccines and immune checkpoint inhibitors, are most effective when their activity is confined to tumor sites