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Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis Total RNA was extracted from cells using ISOGEN (319-90211

Inhibiting S1PR1 effectively prevented the elevated glial fibrillary acidic protein (GFAP) immunoreactivity, morphological changes in astrocytes, increased presynaptic glutamate release, and alterations in cytokine expression

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LNP-based eye drops Three types of LNPs (with nanosizes ranging from 50 to 1,000 nm), namely, liposomes, solid LNPs (SLNs), and nanostructured lipid carriers (NLCs), have emerged as potential nanocarriers for noninvasive delivery of a wide range of drugs [94, 95]