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After interventions, primary hepatocytes were fixed on coverslips for immunofluorescence staining, or scraped from culture dishes and fixed for TEM evaluation, or scraped and stored at 80C for qRT-PCR, Western blot, and GSH and prostaglandin E2 (PGE2) measurements

Oral PBAT-MPs exposure caused marked particle accumulation in intestinal tissue, accompanied by gut microbiota dysbiosis, elevated inflammation, and clear histopathological injury

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