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The pH of the medium was adjusted to pH 3.5 via the addition of cone, ammonium hydroxide

and Fridovich I., Subcellular Distribution of Superoxide Dismutases (SOD) in Rat Liver: Cu,ZnSOD in Mitochondria, Journal of Biological Chemistry 276 (2001): 3838838393, 10.1074/jbc.M105395200