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Hepatic knockdown of GADD45 enhances hepatic lipogenenic gene expression in MCD diet-fed mice To investigate how GADD45 knockdown aggravates hepatic steatosis, we compared the expression of lipogenesis-related genes in the livers of GADD45-knockdown and control mice fed an MCD diet

In detail, anesthesia was induced by intraperitoneal administration of chloral hydrate (400 mg/kg body weight)

The cellular uptake of nanoparticles involves various endocytic pathways, including phagocytosis, macropinocytosis, clathrin- and caveolin-mediated endocytosis

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Under physiological conditions, ferritin-bound or excess Fe 2+ is delivered to lysosomes via NCOA4-mediated ferritinophagy or through direct uptake by transporters such as ZIP8, ZIP14, and DMT1