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Embryology, placenta

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Quantitative real-time PCR Total RNA was extracted from homogenized tissue or cell culture and reverse transcribed as described previously (Schleicher et al, 2016)

Although many studies have shown that GDNF can promote longdistance regeneration of axons after spinal cord injuries, the uncontrolled expression of GDNF may have side effects owing to an excessive axonal growth, aberrant sprouting, and axonal entrapment resulting in a pathological hypertrophy of the injured nerve (Eggers et al., 2019)