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[DOI] [PubMed] [Google Scholar] Kung MP, Kostyniak P, Olson J, Malone M, Roth JA

CRISPR-mediated GPX4 deletion Guide RNAs targeting the first exon of human GPX4 were designed using the CRISPR Design tool (Zhang lab, MIT)

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coli BL21 and enzymatic assay For DsbA in vitro assays, DsbA was expressed as N-terminally truncated variant lacking the periplasmic signal peptide and N-terminally fused to a Strep-Tag with a TEV-cleavage site in E

Furthermore, MGL exerted its protective effects by inhibiting the PI3K/AKT signaling pathway [492] and the arachidonate 5-lipoxygenase (ALOX5)-mediated ferroptosis, inhibiting M1 while promoting M2 macrophages [493]