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near the start, the protease may strip it Glutamine at any position contributes more than its share because P1 Q is the most stringent feature recognized Crystal structure: The TEV S1 pocket is deep and Gln-specific (Phan et al., 2002), but the S2S6 pockets are shallow and water-mediated, explaining the tolerance (Phan et al., 2002)

10.1371/journal.pone.0090104 20

Consequently, knowledge of a model systems ability to handle oxidative stress is highly relevant for the interpretation of results using in vitro model systems