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The cell lysates were centrifuged (15,000 g , 10 min, 4C), and the post-nuclear supernatants were pre-cleared using protein G Sepharose (30 min, 4C)

Drp1 is the main regulator of mitochondrial fission and Fis1 is a partner protein of Drp1 (Losn et al., 2013)

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