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doi: 10.1111/j.1600-079x.2008.00628.x 16 KrsmanoviLZStojilkoviSSMerelliFDufourSVirmaniACattKJ

CD5/ LNP delivered ~6 10 9 copy number of FAPCAR-mRNAs (normalized to GAPDH) to T cells within 24 h (Fig

DNA fiber assay Cells were pulse-labeled with 30 M thymidine analog 5-chloro-2-deoxyuridine (CldU) (Sigma-Aldrich, C6891) for 30 min, then washed three times with PBS (pre-warmed to 37 C), followed by pulse-labeling with 250 M 5-iodo-2-deoxyuridine (IdU) (Sigma-Aldrich, I7125) for 30 min