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In a lactate-rich microenvironment, the number of live cells of hiPSC-VSMC and HA-SMCs increased by about 5565%, and their morphology changed from a regular spindle shape to an irregular flat shape, accompanied by a decrease in contractile markers such as -SMA, SM-MHC, and calponin and an increase in synthetic markers such as vimentin, Collagen I, MMP-9, and OPN [113]

[DOI] [PubMed] [Google Scholar] Sokolovska A

Several studies have demonstrated that in vitro heat stress has detrimental effects on various aspects of cytoplasmic and nuclear oocyte maturation 70 , including cytoskeletal rearrangement 14 , meiotic spindle formation 14,67 and early embryonic mitotic failure 71

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doi: 10.1016/j.nut.2018.05.022 119 RibotJCLopesNSilva-SantosB