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We conducted titration experiments on the complex of 15 N-labeled p53 TAD (13-61) and unlabeled FOXO4 FHD with increasing concentrations of p53 TAD PEP

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For biosynthesis inhibition, 18 M cycloheximide (Sigma-Aldrich) were pre-incubated with cells for 2 hours before adding GrK or GrKSA

Although 5-Amino-1MQ is frequently mentioned with peptides and other substances that improve performance, it is not a peptide in the traditional sense