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Another approach is to use double or multiple strains to construct in vitro biofilms for research

When the cultures reached an OD 600 of 0.40.5, we added 5 mM GGE to them

Candidate Genes and Process in Atherosclerosis

At this stage, metabolic reprogramming not only sustains myocardial injury but also provides a critical metabolic foundation for the progression of myocarditis toward dilated cardiomyopathy (77)