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Biochemical features Biochemical features in ferroptosis involve iron accumulation, lipid peroxidation, generation of ROS, and depletion of glutathione peroxidase 4 (GPX4)

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New findings connect NRF2 not only to an elevated anti-oxidant capacity but also to expression of other types of protective proteins such as brain derived neurotrophic factor (5), the anti-apoptotic B-cell lymphoma 2 (6), the anti-inflammatory interleukin (IL)-10, the mitochondrial transcription (co)-factors NRF-1 and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1) (7), the iron exporter ferroportin 1 (8), and the autophagic protein p62 (9)

To produce the Xkr8 germline KO mice, exons 2 and 3 of the WT Xkr8 allele were deleted using CRISPRCas9 system, as illustrated in Extended Data Fig

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