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For H 2 O 2 treatment, 100 M H 2 O 2 was added to RPMI 1640 growth medium (#1003461059, Sigma Aldrich, United Kingdom), without FBS and phenol red, supplemented with 15 mM HEPES (#1003098482, Sigma Aldrich, USA), 27.9 mM sodium bicarbonate (#101920550, Sigma Aldrich, Japan), 0.2 mM sodium pyruvate (#1002136564, Sigma Aldrich, Japan), 1 U/mL penicillin, and 1 g/mL streptomycin

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While kojic acid is naturally derived, some people prefer exploring other natural skin whitening options