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The exact cause of hot flashes is not yet fully understood, but they are believed to be linked to the decrease in estrogen levels during menopause

Reactions were filled to 200 l with reaction buffer (10 mM Tris-HCl, pH 7.8, 50 mM NaCl, 4 mM MgCl 2 , 8 mM ATP)

(Eur J Endocrinol 1998) : caracterizacin original

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The excised tissue specimens were collected immediately after surgery, with a portion stored in a preservation buffer at -80 C and the remaining formalin-fixed paraffin-embedded