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GSH performs its protective role with the help of two enzymes, glutathione peroxidase and glutathione reductase [1] (Figure 1)

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Using the Tomtec Quadra4, the supernatant was applied to an Oasis HLB 96-well elution plate (30 mg of sorbent per well), the plate was washed with 1 mL of Optima water, and acetyl-CoA was eluted into a deep-well 96-well plate using 1 mL of 25 mM ammonium acetate in methanol