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Disclosures: Prajna Anirvan: Nothing to Disclose, Zaiba Khan: Nothing to Disclose, Pallavi Bhuyan: Nothing to Disclose, Sujata Dixit: Nothing to Disclose, Rishikesh Dash: Nothing to Disclose, Priyanka Mishra: Nothing to Disclose, Giriprasad Venugopal: Nothing to Disclose, Gowri Balachander: Nothing to Disclose, Pankaj Bharali: Nothing to Disclose, Mrinal Gogoi: Nothing to Disclose, Chitta Ranjan Khatua: Nothing to Disclose, Manas Panigrahi: Nothing to Disclose, Manoranjan Ranjit: Nothing to Disclose, Balamurugan Ramadass: Nothing to Disclose, Shivaram Singh: Boehringer Ingelheim International GmbH: Advisor, Sun Pharma: Stock - publicly traded company, Torrent Pharmaceuticals: Stock - publicly traded company, Dr Reddy's Labs: Stock - publicly traded company, Panacea Biotech Ltd: Stock - publicly traded company 606 BIOMARKER RESPONSE IN METABOLIC DYSFUNCTION-ASSOCIATED STEATOHEPATITIS (MASH) PATIENTS WITH HIGH-RISK BASELINE FAST SCORES: OBSERVATIONS FROM THE ENLIVEN PHASE2B TRIAL WITH PEGOZAFERMIN Cynthia Hartsfield 1 Naim Alkhouri 2 Quentin Anstee 3 Rohit Loomba 4 Manal Abdelmalek 5 Kris Kowdley 6 Maya Margalit 1 Mildred Gottwald 1 Germaine Agollah 1 Shibao Feng 1 Hank Mansbach 1 Arun Sanyal 7 , 1 89bio, 2 Arizona Liver Health, 3 Newcastle University, UK, 4 University of California, San Diego, 5 Mayo Clinic Rochester, 6 Liver Institute Northwest, 7 Virginia Commonwealth University Background: ENLIVEN was a 48-week study of the FGF21 analog, pegozafermin (PGZ), in subjects with biopsy-proven F2/F3 MASH

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25 However, most of the subsequent studies focused on the functions of the gut microbiome, fecal microbiome, etc., in the lung