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Immunohistochemistry Mice were perfused with ice-cold PBS, followed by Zambonis fixative

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This critical step prevents condensation from forming inside the vial when bacteriostatic water is added, which can: Introduce uncontrolled water into the reconstitution (altering final concentration) Create localized temperature differentials affecting peptide stability Complicate visual verification of complete reconstitution Step 3 Sterilize the Vial Top and Prepare Bacteriostatic Water Using an alcohol prep pad, thoroughly swab the rubber stopper of both the peptide vial and bacteriostatic water vial