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Description

Zhou, J

Antonini M, Aguzzi C, Fanelli A, Frassineti A, Zeppa L, Morelli MB, et al

Buffers used for the various steps in the solubility of IBs LSB-1: 50 mM TrisCl pH 7.4, 1 mM EDTA, 10 mM -mercaptoethanol, 1% Triton-X 100, 300 mM NaCl, 1 mM Phenylmethanesulfonyl fluoride (PMSF) (Sigma, Catalogue-P7626) and 500 L BugBuster Protein Extraction Reagent (70,584 Merck-Millipore)

10 Dilution Buffer and DCFDA/H 2 DCFDA Cellular ROS Assay Kit were purchased from Abcam (Cambridge, UK)

Glutathionylated and Fe-S cluster containing hMIA40 (CHCHD4) regulates ROS and mitochondrial complex III and IV activities of the electron transport chain