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Cell culture transfection assays Transient cell transfection was carried out with TransIT-X2 Transfection Reagent (Mirus Bio, Madison, WI, USA) with plasmid constructs containing sequences encoding proIGF-1s as previously described 7

Syringe Units = Volume to Draw (mL) 100 = 1.0 mL 100 = 100 units NAD+ Dosing Profile (100 mg/mL Concentration) 50 mg Dose: Draw 50 units (0.5 mL)

doi: 10.1038/s41467-022-31932-x [DOI] [PMC free article] [PubMed] [Google Scholar] 43

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