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and/or (iii) Diagnostic Statistical Manual diagnosis by a physician along with standardized validated questionnaires which have good correspondence to the gold-standard instruments including the Social Responsiveness Scale (SRS) [34, 35], the Social Communication Questionnaire [36,37,38] and the Autism Symptoms Questionnaire [39] and diagnosis confirmation by the Principal Investigator (first author) who specializes in the diagnosis and treatment of children with ASD

By supplementing NAD + precursors or activating the Sirtuin 1 (SIRT1)/AMPK pathway, mitochondrial biogenesis and antioxidant capacity can be synergistically enhanced, correcting immune-metabolic imbalance in both directions and providing an additional molecular target for IBD intervention [458, 459]