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Immunofluorescence staining NPCs were fixed with 4% paraformaldehyde for 20 min, permeabilised with 0.5% Triton X-100 for 15 min and blocked with 5% bovine serum albumin for 30 min

Moreover, it shields the skin from environmental stressors like pollution and UV radiation, which are notorious for inducing premature ageing and damage

HFD differentially methylates vitamin D metabolism enzyme genes and VDR To identify the gene-specific methylation of CpG island of vitamin D metabolism genes and VDR, we adapted a digestion-based gene-specific PCR methylation assay

ACSL1, enriched in LDMCSs, converts FFAs into acyl-CoA

doi: 10.1093/toxsci/kfq375