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Front Pharmacol 9:1193

Moreover, berberine was shown to induce apoptosis in murine BM-DCs and splenic DCs in a dose-dependent manner (from 2 to 50 M) while not affecting other immune cell types such as macrophages, B and T cells (126)

Sample lysates were separated via 414% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), following which the proteins were transferred to polyvinylidene difluoride (PVDF) membranes