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Description

Before LCMS analysis, the dried extracts were reconstituted in 100 L of ACN:H 2 O (1:1, v/v), sonicated for 10 min, and centrifuged for 15 min at 16,200 g and 4 C to remove insoluble debris

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To examine the effect of S-thioallylation on the conformation of metallothionein, 10 M MT2 (5 M in case of ajoene) was incubated with 800 M allicin or 400 M ajoene, and CD spectra were recorded every 10 min (Fig