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Structure of formaldehyde dehydrogenase from Pseudomonas aeruginosa : the binary complex with the cofactor NAD(+)

Insights into eukaryogenesis from the fossil record

Electrophoresis 30 , 276291 (2009)

Single- or double-antigen labeling of formalin-fixed and paraffin-embedded 4-m-thick sections by immunocytochemistry (ICC) or immunohistochemistry (IHC) included the following steps: deparaffinization, rehydratation, boiling in EDTA buffer (1 mM EDTA, 0.05% Tween 20, pH 8.0) for 15 min, 1% H 2 O 2 incubation for 15 min and blocking in SuperBlock solution (ScyTek Laboratories, Logan, UT) for 30 min, followed by primary antibody application

Activity must be appropriately loaded, not either absent or excessive: Complete rest prevents the mechanical stimulus that signals the tendon to rebuild with the appropriate architecture