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Chen C-A, Lin C-H, Druhan LJ, Wang T-Y, Chen Y-R, Zweier JL

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Individual human hairs were pulled out with forceps in the occipital area of the scalp, and plucked hairs with visible bulbs were selected by microscopy

At physiological pH, the cysteine thiol (pKa ~8) is substantially more nucleophilic than the lysine amine (pKa ~10.5), making non-enzymatic acetylation of cysteine thermodynamically and kinetically favored 31