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In contrast, pooled analysis of subjects from the 4.8 and 7.2 g treatment groups showed a 10.47% increase of total glutathione levels at the end of the 2 weeks of GlyNAC supplementation (medians, V2 = 819.7, V4 = 905.4 mg/L per %Ht, p -value = 0.016) (Figure 4C)

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The PVDF membrane was immunoblotted with 2,4-dinitrophenylhydrazone rabbit antibody (dilution 1:150) and secondary anti-rabbit IgG HRP antibody (dilution 1:300) included in the kit

However, it can be stated with a certain degree of confidence that iron overload-dependent cell death of erythrocytes, if it exists, will significantly differ from its counterpart in nucleated cells