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Some of the characteristic symptoms of this deficiency include: extreme tiredness lack of energy breathlessness feeling faint headaches pale skin noticeable heartbeats (palpitations) loss of appetite and weight loss a decline in your mental abilities (brain fog) irritability depression If youre following a vegan diet and recognise any of the symptoms outlined above (or just wish to prevent them!), a vegan B12 supplement may be required and you have two options available

Western blot analysis Total protein extracts from liver tissues and HepG2 cells were prepared using RIPA buffer containing 50 mM Tris-HCl, 150 mM NaCl, 5 mM MgCl2, 2 mM EDTA, 1 mM NaF, 1% NP40, and 0.1% SDS