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W.LeeB.et al

doi: 10.1073/pnas.1004594107 13 KalachikovSMigliazzaACayanisEFracchiollaNSBonaldoMFLawtonLet al

Goldberg, APOE Genotype Modulates Proton Magnetic Resonance Spectroscopy Metabolites in the Aging Brain, Biol

Activities of the control enzymes GSH peroxidase (GPx) and lactate dehydrogenase (LDH) were determined using published methods [22,23] with 1 mM sodium azide, 3.0 units of yeast GR (Sigma), 1 mM GSH, 0.02 mM DTT, 0.0007% (w/v) H 2 O 2 , and 100 M NADPH in the GPx assay and 10 M sodium pyruvate and 100 M NADPH in the LDH assay