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This suppression of the HPA axis can have a protective effect against depression (71, 177, 178)

293,294 This leads to mitochondrial calcium overload, causing cyclophilin D (CypD) to relocate from the mitochondrial matrix to the inner membrane, promoting the formation of the mitochondrial permeability transition pore (mPTP), further inhibiting ATP synthesis, activating oxidative stress, and apoptosis

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Specifically, the acceptable limits were defined as precision (CV) 15% and accuracy (recovery) within 85115% for medium and high concentration levels, with slightly wider limits (CV 20%, recovery 80120%) permitted at the low concentration levels close to the LOQ

Plasmin(ogen) activation assay Activation of glu-plasminogen by uPA and cleavage of the chromogenic substrate D-Val-Leu-Lys- p -nitroanilide dihydrochloride was entirely described previously 40,78