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Tumors derived growth factors like TGF- and platelet-derived growth factor (PDGF) activates the fibroblasts present within the CAFs, which in turn release abundant ECM proteins, leading to desmoplasia with abundant collagen I/III, fibronectin, and other fibrillar matrices ( 4.3 Hypoxia and angiogenesis Uncontrolled division of breast cancer cells often outgrows their blood supply, creating hypoxic conditions (low oxygen level)

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Increased high-temperature requirement for A serine peptidase 1 (HTRA1) levels activate NF-B protein synthesis, whereas HTRA1 knockdown downregulates NF-B protein expression

Describe and interpret the abnormalities in the biochemistry

Rotating injection sites weekly helps prevent lipodystrophy, reduces localized irritation, and maintains consistent absorption