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Functional validation of RNA-seq results To identify various genes that may be involved in the development of dermal fibrosis mediated by NPs in human skin, we performed high-throughput RNA sequencing (RNA-seq) using in vitro HaCaT cells treated with Ag 2 S or Li-doped Ag 2 S NPs at two concentrations (160 and 320 g/mL) for 24 h