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Mandai M, Hamanishi J, Abiko K, Matsumura N, Baba T, Konishi I

Cells were collected by first quenching Trypsin 1:1 (v/v) with supplemented media and transferred to 15 ml Falcon tubes, followed by centrifugation (5 min, 2000 g , 20C)

This impairment is exarcebated by an increase in ROS, despite autophagys critical role in removing these proteins aggregates (Bandyopadhyay and Cuervo, 2007

The other is efflux transporters, which are generally ATP-binding cassette transporters, such as P-glycoprotein and breast cancer resistance protein (BCRP)